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Addgene

p255 pPr-CREM/Ins/CMV-STOP-luc
(Plasmid #8408)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pbeta-gal-Basic
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREM/insulators/lox/luciferase
  • Alt name
    modified Cre/lox
  • Mutation
    modified Cre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRICI (destroyed during cloning)
  • 3′ cloning site DraIII (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chicken globin insulators between Pr-CREM and CMV-STOP-luc cassettes. Pr-CREM from p240 pPr-CREM/Ins isolated with DraIII and Eco47III and ligated into EcoRICI/DraIII fragment of pCMVe-betaAc-STOP-luc (p231).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p255 pPr-CREM/Ins/CMV-STOP-luc was a gift from Jeffrey Green (Addgene plasmid # 8408 ; http://n2t.net/addgene:8408 ; RRID:Addgene_8408)

  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
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