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Addgene

pKS1
(Plasmid #84079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6692
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow on LB agar with kanamycin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetanus neurotoxin fragment C
  • Alt name
    Tetanus fragment C
  • Species
    Synthetic; Clostridium tetani
  • GenBank ID
    AM412776
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHi (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS1 was a gift from Neil Fairweather (Addgene plasmid # 84079 ; http://n2t.net/addgene:84079 ; RRID:Addgene_84079)

  • For your References section:

    Analysis of mutants of tetanus toxin Hc fragment: ganglioside binding, cell binding and retrograde axonal transport properties. Sinha K, Box M, Lalli G, Schiavo G, Schneider H, Groves M, Siligardi G, Fairweather N. Mol Microbiol. 2000 Sep;37(5):1041-51. mmi2091 [pii] PubMed 10972823
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