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Addgene

p240 pPr-CREM/Ins
(Plasmid #8405)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pbeta-gal-Basic
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    probasin driven CREM with insulators
  • Alt name
    modified Cre
  • Mutation
    modified Cre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site DraIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

428 bp probasin promoter fragment from prPb-tva950 (Pam Schwartzenberg, NCI), CREM from p210 pCMV-CREM, pair of chicken globin insulators from plasmid pJC13.1 (G. Felsenfeld, NCI), coding sequence for beta-gal removed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p240 pPr-CREM/Ins was a gift from Jeffrey Green (Addgene plasmid # 8405 ; http://n2t.net/addgene:8405 ; RRID:Addgene_8405)

  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
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