pCAG-Neo-PuroDTK
(Plasmid #84036)

• Purpose: (Empty Backbone) PiggyBac cassette contains Neomycin, puromycin and TK genes
• Depositing Lab: Yuet Wai Kan
• Publication: Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 10.1073/pnas.1407473111. Epub 2014 Jun 9.

Backbone

• Vector backbone: 5'-PTK-3'
• Backbone manufacturer: Wellcome trust Sanger Institute
• Modifications to backbone: Insert CAG promoter, Neomycin gene
• Vector type: Mammalian Expression, Bacterial Expression
• Promoter: CAG promoter
• Selectable markers: Neomycin (select with G418), Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7:taatacgactcactatagg
• 3′ sequencing primer: T3: aattaaccctcactaaagg

Resource Information

• Supplemental Documents:
  • Protocol
• A portion of this plasmid was derived from a plasmid made by: The vector backbone is gifted from Dr. Allan Bradley
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry