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FUW-tetO-wtYAP
(Plasmid #84009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW-tetO-MCS
  • Backbone manufacturer
    Piccolo lab
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 9970
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3, 37ºC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YAP1 (siRNA insensitive)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1540
  • GenBank ID
    NM_001130145.2
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter TRE-CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PshAI (destroyed during cloning)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer pBluescriptKS
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert (FLAG-hYAP1 siRNA insensitive) was generated by PCR from original cDNA kindly provided by Dr. K.L. Guan, and made insensitive to YAP siRNA#1 and siRNA#2 by point mutagenesis (as in Aragona et al., 2013).

YAP siRNA#1 sequence: GACAUCUUCUGGUCAGAGA

YAP siRNA#2 sequence: CUGGUCAGAGAUACUUCUU

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-tetO-wtYAP was a gift from Stefano Piccolo (Addgene plasmid # 84009 ; http://n2t.net/addgene:84009 ; RRID:Addgene_84009)

  • For your References section:

    Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305
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