pUC57-white[coffee]
(Plasmid #84006)

• Purpose: Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
• Depositing Lab: Phillip Zamore
• Publication: Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: GenScript, Inc.
• Backbone size w/o insert (bp): 2517
• Total vector size (bp): 4597
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: A 2,080 bp fragment of the white[coffee] allele
• Alt name: CG2759
• Species: D. melanogaster (fly)
• Insert Size (bp): 2080
• Mutation: A GC-to-AA mutation that creates a G589E missense mutation; silent mutations to confer resistance to white sgRNAs-1, -2, -3 and -4
• Entrez Gene: w (a.k.a. Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel\CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]])

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: M13_F
• 3′ sequencing primer: M13_R

Resource Information

• Supplemental Documents:
  • pUC57-white[coffee] annotated sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry