pLenti-L3US2-RFP-HDAC3
(Plasmid #83966)

• Purpose: Lentiviral CRISPR HDAC3 dual gRNA targeting vector
• Depositing Lab: Eleanor Chen
• Publication: Chen lab CRISPR plasmids (unpublished)

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 5665
• Total vector size (bp): 8492
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: TagRFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HDAC3 dual gRNAs
• gRNA/shRNA sequence: GTTCTGCTCGCGTTACAC, GGGTCAATGCCAGGCGAT
• Species: H. sapiens (human)
• Entrez Gene: HDAC3 (a.k.a. HD3, KDAC3, RPD3, RPD3-2)
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry