GAE teo-GFP
(Plasmid #83958)

• Purpose: Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.
• Depositing Labs: Vincent Lotteau, Philippe Mangeot
• Publication: Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 10.1038/mt.2011.138. Epub 2011 Jul 12.

Backbone

• Vector backbone: Lentiviral vector (SIV derived)
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TetO-GFP
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Can be used to produce inducible SIV-derived vector. Packaging constructs can be derived from HIV-1 without loss of titer and tranduction efficiencies in cell line. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter. This activation can be ensured by a constitutive expression of the tTA in recipient cells or by addition of tTA-gesicles. Expression of GFP is low without activation, depending on the recipient-cell type.
GFP can be replaced using Age1 and BamH1.