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Addgene

mCherry-PAK2 binding domain
(Plasmid #83951)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83951 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    mCherry-C1
  • Backbone size w/o insert (bp) 4645
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pak2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • Mutation
    aa60-113 only; S74A F84A
  • GenBank ID
    NM_002577
  • Entrez Gene
    PAK2 (a.k.a. KNO2, PAK65, PAKgamma)
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • mCherry (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-PAK2 binding domain was a gift from Ryohei Yasuda (Addgene plasmid # 83951 ; http://n2t.net/addgene:83951 ; RRID:Addgene_83951)
  • For your References section:

    Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity. Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, Yasuda R. Nature. 2016 Sep 28;538(7623):104-108. doi: 10.1038/nature19784. 10.1038/nature19784 PubMed 27680697