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PurposeRac1 FLIM donor for dynamic imaging of GTPase activity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEGFP(A206K)-C1
- Backbone size w/o insert (bp) 4684
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameras-related C3 botulinum toxin substrate 1
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Alt nameRac 1
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SpeciesH. sapiens (human)
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Insert Size (bp)579
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
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Tag
/ Fusion Protein
- mEGFP (N terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEGFP-Rac1 was a gift from Ryohei Yasuda (Addgene plasmid # 83950 ; http://n2t.net/addgene:83950 ; RRID:Addgene_83950) -
For your References section:
Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity. Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, Yasuda R. Nature. 2016 Sep 28;538(7623):104-108. doi: 10.1038/nature19784. 10.1038/nature19784 PubMed 27680697