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Addgene

pp-mCAT1
(Plasmid #83948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Total vector size (bp) 6745
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCAT1
  • Alt name
    Ecotropic Receptor
  • GenBank ID
    Ecotropic Receptor NM_007513.4
  • Entrez Gene
    Slc7a1 (a.k.a. 4831426K01Rik, AI447493, Atrc-1, Atrc1, Cat1, Rec-1, Rev-1, mCAT-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pp-mCAT1 was a gift from Vincent Lotteau & Philippe Mangeot (Addgene plasmid # 83948 ; http://n2t.net/addgene:83948 ; RRID:Addgene_83948)

  • For your References section:

    Protein transfer into human cells by VSV-G-induced nanovesicles. Mangeot PE, Dollet S, Girard M, Ciancia C, Joly S, Peschanski M, Lotteau V. Mol Ther. 2011 Sep;19(9):1656-66. doi: 10.1038/mt.2011.138. Epub 2011 Jul 12. 10.1038/mt.2011.138 PubMed 21750535
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