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pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
(Plasmid #83947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p426-SNR52p-gRNA.CAN1.Y-SUP4t
  • Backbone manufacturer
    George Church lab, Dicarlo et al Nucleic Acids Res
  • Vector type
    Yeast Expression
  • Selectable markers
    nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting ADE2 gene
  • gRNA/shRNA sequence
    aattgtagagactatccaca
  • Species
    S. cerevisiae (budding yeast)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX) was a gift from Irina Borodina (Addgene plasmid # 83947 ; http://n2t.net/addgene:83947 ; RRID:Addgene_83947)

  • For your References section:

    CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains. Stovicek V, Borodina I, Forster J. Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 10.1016/j.meteno.2015.03.001. eCollection 2015 Dec. 10.1016/j.meteno.2015.03.001 PubMed 34150504
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