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Addgene

pEQ176
(Plasmid #83943)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEQ3
  • Modifications to backbone
    pEQ3 is a promoterless beta-galactosidase (beta-gal) expression plasmid derived from pON1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CMV enhancer/promoter
  • Species
    CMV
  • Insert Size (bp)
    1100
  • GenBank ID
    X17403
  • Promoter CMV
  • Tag / Fusion Protein
    • beta-galactosidase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEQ176 was a gift from Adam Geballe (Addgene plasmid # 83943 ; http://n2t.net/addgene:83943 ; RRID:Addgene_83943)

  • For your References section:

    Translational control of human cytomegalovirus gp48 expression. Schleiss MR, Degnin CR, Geballe AP. J Virol. 1991 Dec;65(12):6782-9. PubMed 1658374
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