pEQ1007
(Plasmid #83938)

• Purpose: express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells
• Depositing Lab: Adam Geballe
• Publication: Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub 2006 Sep 20.

Backbone

• Vector backbone: pcDNA3.1/V5-His TOPO TA
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRS1
• Species: human cytomegalovirus
• Mutation: R833L in IRS1 (please see depositor comment)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Blp1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation R833L in IRS1 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.