hsDCX-EGFP-pHAT
(Plasmid #83918)

• Purpose: human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II
• Depositing Lab: Gary Brouhard
• Publication: Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi: 10.1016/j.devcel.2012.05.006. Epub 2012 Jun 21.

Backbone

• Vector backbone: pHAT2
• Backbone manufacturer: Peränen J., Rikkonen M.,Hyvönen M., Kääriäinen L. (1996) T7 vectors with modified T7lac promoter for expression of proteins in E
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Doublecortin isoform b
• Alt name: DCX isoform b
• Species: H. sapiens (human); NM_178152.2
• Entrez Gene: DCX (a.k.a. DBCN, DC, LISX, SCLH, XLIS)
• Tags / Fusion Proteins:
  • EGFP+Strep-tag II (C terminal on insert)
  • 6-His (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: EGFP_rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hsDCX cDNA was a gift from Christopher A. Walsh (Boston Children's Hospital/Harvard Medical School)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry