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p169 pCMV/betaAc-EGFP/beta-gal
(Plasmid #8388)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Cre/Lox ; Beta-gal

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    floxed EGFP beta-gal
  • Alt name
    loxI EGFP loxII
  • Alt name
    beta-gal
  • Insert Size (bp)
    4400

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

StuI/SalI fragment from pSV-EGFP/beta-gal cloned into XhoI site of pCAGGS. Floxed EGFP gene switch cassette driven by CMV enhancer/chicken beta-actin promoter. Floxed EGFP cassette can be removed using KpnI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p169 pCMV/betaAc-EGFP/beta-gal was a gift from Jeffrey Green (Addgene plasmid # 8388 ; http://n2t.net/addgene:8388 ; RRID:Addgene_8388)

  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
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