pGP4G-TPLRD1-ccdB
(Plasmid
#83877)
-
Purpose(Empty Backbone) Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 83877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneunknown
-
Vector typeGateway Destination vector
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DB3.1
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNone
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGP4G-TPLRD1-ccdB was a gift from Jennifer Nemhauser (Addgene plasmid # 83877 ; http://n2t.net/addgene:83877 ; RRID:Addgene_83877) -
For your References section:
Recapitulation of the forward nuclear auxin response pathway in yeast. Pierre-Jerome E, Jang SS, Havens KA, Nemhauser JL, Klavins E. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9407-12. doi: 10.1073/pnas.1324147111. Epub 2014 Jun 16. 10.1073/pnas.1324147111 PubMed 24979769