pDONR-IAA3_no stop
(Plasmid
#83863)
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PurposeGateway donor vector for IAA3 with no stop codon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
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Vector typeGateway Donor vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIAA3
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Alt nameSHY2
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SpeciesA. thaliana (mustard weed)
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Mutationno stop codon
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Entrez GeneSHY2 (a.k.a. AT1G04240, F19P19.32, F19P19_32, IAA3, SHORT HYPOCOTYL 2, indole-3-acetic acid inducible 3)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR-IAA3_no stop was a gift from Jennifer Nemhauser (Addgene plasmid # 83863 ; http://n2t.net/addgene:83863 ; RRID:Addgene_83863) -
For your References section:
A synthetic approach reveals extensive tunability of auxin signaling. Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins E, Nemhauser JL. Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. Epub 2012 Jul 27. 10.1104/pp.112.202184 PubMed 22843664