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p144 pSV-EGFP/beta-gal
(Plasmid #8386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV-beta-Galactosidase
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6800
  • Vector type
    Mammalian Expression, Cre/Lox ; Beta-gal

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    floxed EGFP
  • Alt name
    loxI EGFP loxII
  • Insert Size (bp)
    1100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Floxed EGFP gene switch cassette (KpnI fragment
from plasmid "p142") driven by SV40 promoter.

Note that a there is a second Kpn1 site within beta gal resulting in KpnI digests that yield bands of 6kb, 1.1kb, and approximately .6kb.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p144 pSV-EGFP/beta-gal was a gift from Jeffrey Green (Addgene plasmid # 8386 ; http://n2t.net/addgene:8386 ; RRID:Addgene_8386)

  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
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