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pPJ169(JPUB_006769)
(Plasmid #83835)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83835 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbE0a_mut, (colE1 ori, ampR, BsaI site removed through mutagenesis)
  • Selectable markers
    Kan

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Medium Copy
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Kuste3340
  • Alt name
    ACP
  • Insert Size (bp)
    252
  • Promoter op5_BsaI_3340_for

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccacctgaattcatgagatctggatcggtctcaatgcatgataccattcaaaaactgaaagaactgc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Kuste3350
  • Alt name
    ACP
  • Insert Size (bp)
    201
  • Promoter op5_3350_for

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPJ169(JPUB_006769) was a gift from Harry Beller (Addgene plasmid # 83835 ; http://n2t.net/addgene:83835 ; RRID:Addgene_83835)

  • For your References section:

    Investigation of Proposed Ladderane Biosynthetic Genes from Anammox Bacteria by Heterologous Expression in E. coli. Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling JD, Beller HR. PLoS One. 2016 Mar 14;11(3):e0151087. doi: 10.1371/journal.pone.0151087. eCollection 2016. PONE-D-16-02052 [pii] PubMed 26975050
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