pPJ173(JPUB_006777)
(Plasmid #83834)

• Purpose: Carries NEW operon 10 final
• Depositing Lab: Harry Beller
• Publication: Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. doi: 10.1371/journal.pone.0151087. eCollection 2016.

Backbone

• Vector backbone: pBbE0k backbone (colE1 ori, kanR)
• Selectable markers: Kan

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Medium Copy
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Kuste3336
• Alt name: Phyotene desaturase
• Insert Size (bp): 1455
• Promoter: op10_BsaI_3336_for

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aattccgaattcatgagatctggatcggtctcaatgaaagattttgatgtgattgttatcgg

Gene/Insert 2

• Gene/Insert name: Kuste3339
• Alt name: FabZ
• Insert Size (bp): 441
• Promoter: op10_3339_for

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: catttaaaggaggttttctaatgaaaaaagcctttccaagtcc

Gene/Insert 3

• Gene/Insert name: Kuste3341
• Alt name: FabG
• Insert Size (bp): 720
• Promoter: op10_3341_for

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cattaaaggtaccacctaaaggaggttttctaatgctggttacgggtggtag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry