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Addgene

pGEM3/cGata1
(Plasmid #83829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2867
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gata1
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    4100
  • Entrez Gene
    GATA1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM3/cGata1 was a gift from Douglas Engel (Addgene plasmid # 83829 ; http://n2t.net/addgene:83829 ; RRID:Addgene_83829)

  • For your References section:

    Activity and tissue-specific expression of the transcription factor NF-E1 multigene family. Yamamoto M, Ko LJ, Leonard MW, Beug H, Orkin SH, Engel JD. Genes Dev. 1990 Oct;4(10):1650-62. PubMed 2249770
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