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Addgene

RSV/mGATA3 (antisense)
(Plasmid #83817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RSV-CAT
  • Backbone manufacturer
    Gorman, et al. 1982
  • Modifications to backbone
    CAT was removed
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GATA3-antisense strand
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gata3 (a.k.a. Gata-3, jal)
  • Promoter RSV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pREP-F (GCTCGATACAATAAACGCC)
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RSV/mGATA3 (antisense) was a gift from Douglas Engel (Addgene plasmid # 83817 ; http://n2t.net/addgene:83817 ; RRID:Addgene_83817)

  • For your References section:

    Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD. Mol Cell Biol. 1991 May . 11(5):2778-84. PubMed 2017177
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