RSV/mGATA3 (sense)
(Plasmid
#83816)
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PurposeMammalian expression of mouse GATA3
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRSV-CAT
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Backbone manufacturerGorman, et al. 1982
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Modifications to backboneCAT was removed
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGATA3
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SpeciesM. musculus (mouse)
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Entrez GeneGata3 (a.k.a. Gata-3, jal)
- Promoter RSV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pREP-F (GCTCGATACAATAAACGCC)
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RSV/mGATA3 (sense) was a gift from Douglas Engel (Addgene plasmid # 83816 ; http://n2t.net/addgene:83816 ; RRID:Addgene_83816) -
For your References section:
Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD. Mol Cell Biol. 1991 May . 11(5):2778-84. PubMed 2017177