recoverin2b
(Plasmid
#83815)
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Purposeexpress zebrafish recoverin2b
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3973
- Total vector size (bp) 4549
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerecoverin2b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)576
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GenBank IDKT325593
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Entrez Genercvrn3 (a.k.a. rcv2b, zgc:73075)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CACTCAGACAGAAGTCAT
- 3′ sequencing primer GTAGACCATCATCGCTTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
recoverin2b was a gift from Stephan Neuhauss (Addgene plasmid # 83815 ; http://n2t.net/addgene:83815 ; RRID:Addgene_83815) -
For your References section:
Recoverin depletion accelerates cone photoresponse recovery. Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC. Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086. 10.1098/rsob.150086 PubMed 26246494