recoverin2a
(Plasmid
#83813)
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Purposeexpress zebrafish recoverin2a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3973
- Total vector size (bp) 4555
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerecoverin2a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)582
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GenBank IDKT325592
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Entrez Genercvrn2 (a.k.a. fk53a03, rcv1, rcv2a, wu:fk53a03, zgc:73121)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CAACATCTTTCTGAGCCC
- 3′ sequencing primer ATAGCGTCTTCATTCTCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
recoverin2a was a gift from Stephan Neuhauss (Addgene plasmid # 83813 ; http://n2t.net/addgene:83813 ; RRID:Addgene_83813) -
For your References section:
Recoverin depletion accelerates cone photoresponse recovery. Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC. Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086. 10.1098/rsob.150086 PubMed 26246494