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Addgene

recoverin1a
(Plasmid #83811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3973
  • Total vector size (bp) 4585
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    recoverin1a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    612
  • Mutation
    *(see below)
  • GenBank ID
    KT325590
  • Entrez Gene
    rcvrna (a.k.a. rcv1a, zgc:114180)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GGACCAGAGTACAATTTAAG
  • 3′ sequencing primer GAAGCTCTAATCAGTCATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note: Addgene's quality control sequencing identified D82G & M177T amino acid residue substitutions. The effects of these residue changes is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    recoverin1a was a gift from Stephan Neuhauss (Addgene plasmid # 83811 ; http://n2t.net/addgene:83811 ; RRID:Addgene_83811)

  • For your References section:

    Recoverin depletion accelerates cone photoresponse recovery. Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC. Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086. 10.1098/rsob.150086 PubMed 26246494
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