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Addgene

sgRNA 2 GAPDH
(Plasmid #83809)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MLM3636
  • Backbone size w/o insert (bp) 2278
  • Total vector size (bp) 2289
  • Modifications to backbone
    The oligos with overhang were synthesized, annealed and ligated into the sgRNA backbone linearized with BsmB1.
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SgRNA
  • gRNA/shRNA sequence
    AGCCCCAGCAAGAGCACAAG (AGG)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgRNA 2 GAPDH was a gift from Bo Feng (Addgene plasmid # 83809 ; http://n2t.net/addgene:83809 ; RRID:Addgene_83809)

  • For your References section:

    Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, Feng B. Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4. 10.1093/nar/gkw064 PubMed 26850641
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