pEQ1180
(Plasmid
#83805)
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Purposeexpress human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/V5-His TOPO TA
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRS1
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SpeciesHuman CMV
- Promoter CMV
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Tag
/ Fusion Protein
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEQ1180 was a gift from Adam Geballe (Addgene plasmid # 83805 ; http://n2t.net/addgene:83805 ; RRID:Addgene_83805) -
For your References section:
Species specificity of protein kinase r antagonism by cytomegalovirus TRS1 genes. Child SJ, Brennan G, Braggin JE, Geballe AP. J Virol. 2012 Apr;86(7):3880-9. doi: 10.1128/JVI.06158-11. Epub 2012 Jan 25. 10.1128/JVI.06158-11 PubMed 22278235