pDH22
(Plasmid #83798)

• Purpose: Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection
• Depositing Lab: Eric Muller
• Publication: Hailey et al Methods Enzymol. 2002;351:34-49.

Backbone

• Vector backbone: pyGFP
• Backbone manufacturer: Prein et al. 2000, PMID 11086160
• Modifications to backbone: converted GFP to YFP via mutations.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html