pBS10
(Plasmid
#83795)
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PurposeUseful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS4 (Addgene plasmid 83793)
- Backbone size w/o insert (bp) 4875
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Modifications to backboneCFP has been mutated to the Cerulean variant
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCerulean
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS10 was a gift from Eric Muller (Addgene plasmid # 83795 ; http://n2t.net/addgene:83795 ; RRID:Addgene_83795) -
For your References section:
Fluorescence resonance energy transfer using color variants of green fluorescent protein. Hailey DW, Davis TN, Muller EG. Methods Enzymol. 2002;351:34-49. PubMed 12073355
