pBS4
(Plasmid #83793)

• Purpose: Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection
• Depositing Lab: Eric Muller
• Publication: Hailey et al Methods Enzymol. 2002;351:34-49.

Backbone

• Vector backbone: pDH3 (Addgene plasmid 83776 )
• Backbone manufacturer: Muller Lab
• Backbone size w/o insert (bp): 4875
• Modifications to backbone: The kanamycin resistance gene in pDH3 is replaced with the hygromycin B resistance gene.
• Vector type: Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html