pDH3
(Plasmid
#83789)
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PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-GFP(S65T)-HIS
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Backbone manufacturerWach, et al. 1997, PMID 9290211
- Backbone size w/o insert (bp) 4882
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCFP
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MutationF64L, Y66W, N146I, M153T, V163A, N164H mutations added. Also includes S65T and Q80R from parent vector. Q80R is believed to be a neutral mutation.
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDH3 was a gift from Eric Muller (Addgene plasmid # 83789 ; http://n2t.net/addgene:83789 ; RRID:Addgene_83789) -
For your References section:
Fluorescence resonance energy transfer using color variants of green fluorescent protein. Hailey DW, Davis TN, Muller EG. Methods Enzymol. 2002;351:34-49. PubMed 12073355
Map uploaded by the depositor.
