Skip to main content
Addgene

pLMB712
(Plasmid #83784)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIJ11268
  • Backbone size w/o insert (bp) 16260
  • Total vector size (bp) 17427

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli S17-1
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
  • Species
    Rhizobium leguminosarum bv. viciae, strain 3841
  • Insert Size (bp)
    1167

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CCATCTTTGCCCTACCGTAT
  • 3′ sequencing primer AAACCGACGCCATCACCCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLMB712 was a gift from Philip Poole (Addgene plasmid # 83784 ; http://n2t.net/addgene:83784 ; RRID:Addgene_83784)

  • For your References section:

    Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root Secretion. Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, Poole PS. Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. Epub 2017 May 11. 10.1104/pp.16.01302 PubMed 28495892
Related items:
Commonly requested with: