pCasperAUG-Gal4-X
(Plasmid #8378)

• Depositing Lab: Leslie Vosshall
• Publication: Vosshall et al Cell 2000 Jul 21;102(2):147-59.

Backbone

• Vector backbone: pCasper
• Backbone manufacturer: Public Domain--Carl Thummel
• Backbone size w/o insert (bp): 9000
• Vector type: Drosophila P-element vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Yeast Gal4
• Species: S. cerevisiae (budding yeast); fused to first ~30 amino acids of Drosophila ADH
• Insert Size (bp): 3000
• GenBank ID: K01486
• Entrez Gene: GAL4 (a.k.a. YPL248C, GAL81)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, Asp718I*, NotI, BamHI, BglII, PmeI, Asp718* (not destroyed)
• 5′ sequencing primer: gggttttattaacttacat
• 3′ sequencing primer: taagtcggcaaatatcgc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid was constructed by deleting B-Gal gene from pCasperAUG-Bgal from Carl Thummel/Univ. of Utah and replacing with the coding region of Gal4 from the GL83 plasmid provided by Gary Struhl/Columbia University
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry