pDH6
(Plasmid #83777)

• Purpose: Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection
• Depositing Lab: Eric Muller
• Publication: Hailey et al Methods Enzymol. 2002;351:34-49.

Backbone

• Vector backbone: pDH3 (Addgene plasmid 83776 ) and pDH5 (Addgene Plasmid 83775)
• Backbone manufacturer: Muller Lab
• Backbone size w/o insert (bp): 4726
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP
• Species: Synthetic
• Mutation: S65G, V68L, Q69K, S72A, T203Y mutations added.

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html