pDH5
(Plasmid #83775)

• Purpose: Useful for tagging yeast proteins with C-terminal YFP tag; his selection
• Depositing Lab: Eric Muller
• Publication: Hailey et al Methods Enzymol. 2002;351:34-49.

Backbone

• Vector backbone: pFA6a-GFP(S65T)-HIS
• Backbone manufacturer: Wach, et al. 1997, PMID 9290211
• Backbone size w/o insert (bp): 4726
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP
• Mutation: S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R from parent vector. Q80R is believed to be a neutral mutation.

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html