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Addgene

pGEM-rat Fcepsilon RI gamma
(Plasmid #8375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM 3Zf-
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3199
  • Vector type
    Bacterial Expression ; Beta-gal

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rat Fc epsilon RI gamma cDNA
  • Alt name
    FcerIg rat
  • Alt name
    Fc receptor gamma chain rat
  • Alt name
    high affinity receptor IgE rat
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    700
  • GenBank ID
    L04306
  • Entrez Gene
    Fcer1g

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-rat Fcepsilon RI gamma was a gift from Jean Kinet (Addgene plasmid # 8375 ; http://n2t.net/addgene:8375 ; RRID:Addgene_8375)

  • For your References section:

    Complete structure and expression in transfected cells of high affinity IgE receptor. Blank U, Ra C, Miller L, White K, Metzger H, Kinet JP. Nature 1989 Jan 12;337(6203):187-9. 10.1038/337187a0 PubMed 2521376
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