pGEM-rat FceRI beta cDNA
(Plasmid
#8374)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM 4
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2871
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat Fc epsilon RI beta chain
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Alt nameFcer1b rat
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Alt namehigh affinity receptor beta subunit rat
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1051
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GenBank IDM22923
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Entrez GeneMs4a2 (a.k.a. FCIGA, Fcer1b, Ms4a1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-rat FceRI beta cDNA was a gift from Jean Kinet (Addgene plasmid # 8374 ; http://n2t.net/addgene:8374 ; RRID:Addgene_8374) -
For your References section:
Isolation and characterization of cDNAs coding for the beta subunit of the high-affinity receptor for immunoglobulin E. Kinet JP, Blank U, Ra C, White K, Metzger H, Kochan J. Proc Natl Acad Sci U S A 1988 Sep;85(17):6483-7. 10.1073/pnas.85.17.6483 PubMed 2970642