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pGEM-rat FceRI alpha
(Plasmid #8373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM 3Zf-
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3199
  • Vector type
    Bacterial Expression ; Beta-gal

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rat Fc epsilon RI alpha cDNA
  • Alt name
    high affinity receptor I for IgE alpha chain
  • Alt name
    Fcer1a rat
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    811
  • GenBank ID
    M17153
  • Entrez Gene
    Fcer1a (a.k.a. Iger01, RATIGER01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-rat FceRI alpha was a gift from Jean Kinet (Addgene plasmid # 8373 ; http://n2t.net/addgene:8373 ; RRID:Addgene_8373)

  • For your References section:

    A cDNA presumptively coding for the alpha subunit of the receptor with high affinity for immunoglobulin E. Kinet JP, Metzger H, Hakimi J, Kochan J. Biochemistry 1987 Jul 28;26(15):4605-10. 10.1021/bi00389a002 PubMed 2959318
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