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pGEM-hu FceRI beta
(Plasmid #8366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM 3Zf-
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3199
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Fc epsilon RI beta cDNA
  • Alt name
    high affinity receptor for IgE beta chain
  • Alt name
    membrane-spanning 4 domains
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    760
  • GenBank ID
    M89796
  • Entrez Gene
    MS4A2 (a.k.a. APY, ATOPY, FCER1B, FCERI, IGEL, IGER, IGHER, MS4A1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-hu FceRI beta was a gift from Jean Kinet (Addgene plasmid # 8366 ; http://n2t.net/addgene:8366 ; RRID:Addgene_8366)

  • For your References section:

    The gene and cDNA for the human high affinity immunoglobulin E receptor beta chain and expression of the complete human receptor. Kuster H, Zhang L, Brini AT, MacGlashan DW, Kinet JP. J Biol Chem 1992 Jun 25;267(18):12782-7. PubMed 1535625
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