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Addgene

FLAG-FOXO3a TM
(Plasmid #8361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8361 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXO3a
  • Alt name
    FKHRL1
  • Alt name
    forkhead family
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2038
  • Mutation
    T32A, S253A, S315A
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See FLAG-FOXO3a WT for wild-type map and sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-FOXO3a TM was a gift from Michael Greenberg (Addgene plasmid # 8361 ; http://n2t.net/addgene:8361 ; RRID:Addgene_8361)

  • For your References section:

    Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP, Gygi SP, Sinclair DA, Alt FW, Greenberg ME. Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19. 10.1126/science.1094637 PubMed 14976264
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