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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGADD45 promoter P-2/3m
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SpeciesH. sapiens (human)
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MutationP-2/3m
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Entrez GeneGADD45A (a.k.a. DDIT1, GADD45)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Core consensus sequence AACA of FHRE-2 within the GADD45A promoter replaced with GGGG; AC of FHRE-3 replaced with GG.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GADD45 P-2/3m-luc was a gift from Michael Greenberg (Addgene plasmid # 8359 ; http://n2t.net/addgene:8359 ; RRID:Addgene_8359) -
For your References section:
DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS, Chiang LW, Greenberg ME. Science 2002 Apr 19;296(5567):530-4. 10.1126/science.1068712 PubMed 11964479