pBiex PKC - Peptide 14 mCit mCer
(Plasmid #83565)

• Purpose: Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
• Depositing Lab: Sivaraj Sivaramakrishnan
• Publication: Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.737601.

Backbone

• Vector backbone: pBiex-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5259
• Total vector size (bp): 8151
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKC alpha
• Alt name: PRKCA
• Species: H. sapiens (human)
• Insert Size (bp): 2892
• Mutation: Only catalytic domain of PKC (aa 335-672)
• Entrez Gene: PRKCA (a.k.a. AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA)
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • mCitrine
  • mCerulean
  • Tev Protease Site
  • 10 nm ER/K Helix
  • FLAG Purificaiton Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 Foward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry