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Addgene

pILGFPU0 (R)
(Plasmid #83562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; kluyveromyces lactis in S. cerevisiae

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    alpha-Select from BIOLINE (Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
  • Species
    S. cerevisiae (budding yeast)
  • Promoter TEF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer yGFP-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pILGFPU0 (R) was a gift from Claudia Vickers (Addgene plasmid # 83562 ; http://n2t.net/addgene:83562 ; RRID:Addgene_83562)

  • For your References section:

    A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae. Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers CE. Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8. 10.1016/j.ymben.2016.12.003 PubMed 27939849
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