GADD45 WT-luc
(Plasmid #8356)

• Depositing Lab: Michael Greenberg
• Publication: Tran et al Science 2002 Apr 19;296(5567):530-4.

Backbone

• Vector backbone: pGL3
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GADD45 promoter WT
• Species: H. sapiens (human)
• Entrez Gene: GADD45A (a.k.a. DDIT1, GADD45)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry