pILGFPQ4
(Plasmid #83559)

• Purpose: Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.
• Depositing Lab: Claudia Vickers
• Publication: Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 10.1186/s13068-017-0728-x. eCollection 2017.

Backbone

• Vector backbone: pUC19
• Vector type: Yeast Expression
• Selectable markers: URA3 ; kluyveromyces lactis in S. cerevisiae

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): alpha-Select
• Growth instructions: alpha-Select from BIOLINE (Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAL7 promoter-yEGFP
• Species: S. cerevisiae (budding yeast)
• Promoter: GAL7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: yGFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.