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Addgene

pILGFPH7
(Plasmid #83554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; kluyveromyces lactis in S. cerevisiae

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    alpha-Select
  • Growth instructions
    alpha-Select from BIOLINE (Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADH2 promoter-yEGFP
  • Species
    S. cerevisiae (budding yeast)
  • Promoter ADH2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer yGFP-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pILGFPH7 was a gift from Claudia Vickers (Addgene plasmid # 83554 ; http://n2t.net/addgene:83554 ; RRID:Addgene_83554)

  • For your References section:

    Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities. Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5. 10.1186/s12934-015-0278-5 PubMed 26112740
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