HA-FOXO3a WT-ER
(Plasmid #8355)

• Depositing Lab: Michael Greenberg
• Publication: Tran et al Science 2002 Apr 19;296(5567):530-4.

Backbone

• Vector backbone: pECE
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FOXO3a
• Alt name: FKHRL1
• Alt name: forkhead family
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: mouse estrogen receptor (G525R) on C-term
• Entrez Gene: FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
• Tag / Fusion Protein:
  • HA (YDVPDYASL) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Has ER--ligand binding domain of estrogen receptor.
See author's map and sequence.