PHBA03
(Plasmid
#83537)
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PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS406
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
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SpeciesS. cerevisiae (budding yeast)
- Promoter pTEF1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13pUC-Fwd
- 3′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHBA03 was a gift from Claudia Vickers (Addgene plasmid # 83537 ; http://n2t.net/addgene:83537 ; RRID:Addgene_83537) -
For your References section:
The Saccharomyces cerevisiae pheromone-response is a metabolically active stationary phase for bio-production. Williams TC, Peng B, Vickers CE, Nielsen LK. Metab Eng Commun. 2016 May 11;3:142-152. doi: 10.1016/j.meteno.2016.05.001. eCollection 2016 Dec. 10.1016/j.meteno.2016.05.001 PubMed 29468120